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CFTR purified from a solubilized membrane fraction. Elution from a nickel resin (1 ml resin per billion cells starting material) was followed by concentration with an Akta chromatography system (Materials and Methods). Coomassie Blue staining is shown in (A). Western blotting (B) with 1104 antibody (raised against the R domain of CFTR) confirmed that the major band in the Coomassie Blue-stained gel is CFTR.
