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Mass spectrometry analysis. Summary of PTMs identified in CFTR: Phosphorylation: S660, S686, S700, S712, T717, S737, S795, S1444, S1456. Ubiquitination: K688. Palmitoylation: C524, C1395. Methylation: K698, N699, Q744, T757. Gray letters represent residues not covered by proteolysis. Note: All modifications were identified by MS/MS except for palmitoylation of C524 (identified by MRM-MS).
