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Western blot analysis of YFP–Bcl-xL expression in B cells. Equal amounts of total cellular protein (25 µg) from stable pools of B cells expressing YFP or YFP–Bcl-xL were loaded in each lane and subjected to SDS–PAGE and western blot analysis with an anti-Bcl-xL antibody. The expected molecular mass of YFP–Bcl-xL is 54 kDa.
