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Western blot expression of Bcl-xL in Ramos B cells expressing the YFP or YFP–Bcl-xL protein. Equal amounts of total cellular protein (50 µg) from Ramos B cells from the indicated round (R) of the selection process were loaded in each lane to ensure that band intensities reflect actual relative expression levels in the cells. Samples were subjected to SDS–PAGE and western blot analysis with an anti-Bcl-xL antibody.
