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. 2011 Dec 9;25(1):27–38. doi: 10.1093/protein/gzr052

Table II.

Mutations in the YFP–Bcl-xL gene region of transduced Ramos B cells following round 3

graphic file with name GZR052IM2.jpg

The YFP–Bcl-xL gene from genomic DNA of a pool of Ramos B cells was PCR amplified, cloned and sequenced. The sequencing data for 23 different constructs isolated from bacterial colonies are shown. The location and nature of the nucleotide (nt.) mutation is shown as well as the resulting amino acid changes (amino acid location refers to residue within the YFP or Bcl-xL protein). Silent (sil) mutations are also shown.