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. 2011 Apr 22;23(1):245–250. doi: 10.1093/annonc/mdr153

Figure 1.

Figure 1.

(A) Gel electrophoresis showing the detection of PCR products for epithelial cell adhesion molecule (EpCAM), CD49f (α6-integrin), CD117 (c-kit receptor), CD24, CD29 (β1-integrin), and the housekeeping gene cyclophilin from umbilical cord blood-derived mononuclear cells (MNC) from five umbilical cord blood samples (D333, D341, D342, D344, and D349). Water (last lane) was used as negative controls. (B) Double-labeled immunofluorescent confocal microscopy of umbilical cord blood-derived MNC from sample D321 showing colocalization in the overlay image of EpCAM (green) and CD49f (red) (top panel); CD24 (green) and CD49f (red) (middle panel); and CD24 (green) and CD29 (red) (bottom panel). Scale bar represents 20 μm. (C) Flow cytometric pseudocolor plots showing the detection of the EpCAM+, EpCAM+CD49f+, EpCAM+CD49f+CD117+, CD49f+CD24+, CD24+CD29+, and CD49f+CD24+CD29+ subpopulations (boxed, with percentage of cells indicated) from umbilical cord blood-derived MNC of sample N41. The arrows indicate that the triple positive population was derived from the double positive population as shown. The markers shown in the top panel have been reported in humans while the markers shown in the bottom panel have been reported in mice.