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. 2011 Nov 9;33(1):68–76. doi: 10.1093/carcin/bgr246

Fig. 1.

Fig. 1.

Changes in cellular growth, CSCs markers and miR-21 levels in colon cancer CR and/or parental cells. (A) While the growth of parental (P) colon cancer HCT-116 and HT-29 is inhibited, CR colon cancer cells display induction of the same in response to 5-FU (250 μM) + Ox (6.25 μM) in DMEM/10% FBS over a period of 4 days. (B) Western blot showing stimulation of CSCs marker CD44 and reduction of the pro-apoptotic protein PDCD4 in CR-HCT-116 and CR-HT-29 colon cancer cells, when compared with the corresponding parental (P) cells. β-Actin was used as loading controls. The numbers represent percent of corresponding control normalized to β-actin. (C) Quantitative real-time polymerase chain reaction (RT–PCR) showing upregulation of pri-microRNA-21 and mature microRNA-21 in CR-HCT-116 cells and CR-HT-29 cells, when compared with the parental cells (*P < 0.001).