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. 2011 Nov 9;33(1):68–76. doi: 10.1093/carcin/bgr246

Fig. 3.

Fig. 3.

miR-21 overexpression activates β-catenin/TCF/LEF activity (A) Western blot showing the expression of different members (axin, β-catenin, c-Myc, Cyclin-D1) of Wnt/β-catenin signaling pathway in miR-21 expressing clones from HCT-116 cells, stably transfected with pCMV-miR-21 plasmid or the corresponding vector. The numbers represent percent of corresponding control normalized to β-actin. (B) miR-21 overexpression resulted in the activation of TCF/LEF activity in the miR-21 pooled clones cells. (C) Representative photographs showing colonospheres formed by the cells from miR-21 expressing clones, derived from HCT-116 cells, stably transfected with pCMV-miR-21 plasmid or the corresponding vector. (D) Western blot showing the expression of β-catenin after downregulation of miR-21, β-catenin and TGFβR2 in the miR-21-overexpressing cells (pooled clones) by anti-miR-21, β-catenin siRNA or TGFβR2 siRNA. (E) Downregulation of β-catenin using β-catenin siRNA inhibits transcriptional activity of TCF/LEF in the clones (pooled) from HCT-116 cells, stably transfected with pCMV-miR-21 plasmid. The corresponding controls were similarly treated with non-targeted siRNA (control). *P < 0.001, compared with the corresponding vector-treated controls.