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. 2009 Mar 30;24(9):1598–1607. doi: 10.1359/jbmr.090313

FIG. 3.

FIG. 3

Osteoblasts and osteoclasts increase in vertebrae 18 days after AdShhN treatment. (A) Immunohistochemical staining for procollagen I, a cytoplasmic marker of osteoblasts: PBS (left); AdNull (middle); and AdShhN (right). Brown indicates positive staining. Bar = 40 μm. (B) Immunohistochemical staining for cathepin K, a marker of active osteoclasts: PBS (left); AdNull (middle); and AdShhN (right). Bar = 40 μm. (C–E) Quantification of osteoblast and osteoclast numbers (n = 5 for all groups). (C) Osteoblasts/mm of trabecular area. (D) Osteoclasts/mm of trabecular area. (E) Osteoblast/osteoclast ratio. For C–E, data shown as mean ± SE.