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Mitochondrial Bak localization. KB-3 cells were untreated or treated with 30 nM vinblastine for the times indicated. A, Cytosolic (C) and mitochondrial (M) fractions were prepared and subjected to immunoblotting for Bak, Cox II (Complex IV), and procaspase 3, as indicated. B, Whole cell extracts were prepared and subjected to immunoblotting for PARP and GAPDH as a loading control. Uncleaved (116 kDa) and cleaved (85 kDa) PARP species are indicated.
