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. Author manuscript; available in PMC: 2012 Feb 9.
Published in final edited form as: Mol Cancer Ther. 2008 Jul;7(7):2224–2232. doi: 10.1158/1535-7163.MCT-07-2299

Figure 2.

Figure 2

Figure 2

A. Vinblastine induces oligomerization of Bak. KB-3 cells were treated with vinblastine (VBL, 30 nM) for the times indicated, permeabilized with digitonin, and CHAPS solubilized particulate fractions were subjected to SDS-PAGE in the absence or presence of 1% β-mercaptoethanol (β-ME). Immunoblotting for Bak was performed with the monomeric and oligomeric forms indicated. The asterisk denotes the intrachain crosslink conformer of inactive Bak. Molecular mass standards are indicated on the right. B. Overexpression of Bcl-xL inhibits vinblastine-induced Bak oligomerization. KB-3 and two independent KB3-HA-Bcl-xL expressing stable clones were untreated or treated with 30 nM vinblastine (KB-3 cells) or 100 nM vinblastine (KB3-HA-Bcl-xL cells) as indicated, permeabilized with digitonin, and CHAPS solubilized particulate fractions were subjected to SDS-PAGE in the absence of reducing agent. Immunoblotting was performed for Bak and the monomeric and oligomeric forms are indicated. Molecular mass standards are indicated on the right.