Skip to main content
. Author manuscript; available in PMC: 2012 Feb 9.
Published in final edited form as: Mol Cancer Ther. 2008 Jul;7(7):2224–2232. doi: 10.1158/1535-7163.MCT-07-2299

Figure 3.

Figure 3

Figure 3

Vinblastine induces Bak activation and Bak-Bax interaction. A, KB-3 cells were treated or untreated with vinblastine (VBL, 30 nM) for the times indicated and subjected to immunoprecipitation with anti-Bak (NT) antibody followed by immunoblotting for Bak (lanes 2–5, upper panel). Sample precipitated in the absence of NT antibody (lane 6) and a whole cell extract (lane 1) were also examined as controls. Immunoblotting of IgG was used as an additional control for immunoprecipitation (lanes 2–5, lower panel). B, KB-3 cells were untreated or treated with vinblastine (VBL, 30 nM) for the times indicated and subjected to immunoprecipitation with anti-Bax 6A7 antibody (lanes 2–4), followed by immunoblotting for Bax or Bak. Precipitates prepared in the absence of 6A7 antibody (lane 1) and whole cell extracts (lanes 5–7) were also examined as controls. Immunoblotting of IgG was used as an additional control for immunoprecipitation (lanes 2–4, lower panel).