Figure 2.
Spleen leukocytes from CX3CR1−/− mice fail to migrate to RSV G protein or CX3CL1 but not to CCL5. (A) Chemotaxis of leukocytes from CX3CR1−/− (white bars) and wildtype (WT, black bars) C57BL/6 mice to recombinant CX3CL1, purified RSV G protein, and CCL5 (RANTES) was assessed. (B) To inhibit CX3CL1- and RSV G–protein-mediated cell chemotaxis, antiCX3CR1 antibody and antiRSV G monoclonal antibody (131-2G) were added to chambers containing either CX3CL1 or RSV G protein, and the percentage inhibition of chemotaxis was determined. *, P < 0.05; n/d, not detected.