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. 2012 Jan 3;11:1. doi: 10.1186/1475-2859-11-1

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Copyright ©2012 Adamo et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fluorimetric analysis of superoxide anion (OH^-•) production. Evolved and non-evolved C. humilis (a) and S. cerevisiae (b) cells exponentially growing in YPD (white bars) and in YPD + 2.5 g ^.L^-1CuSO₄(grey bars). OH^-•formation is expressed as fluorescence intensity of ethidium in arbitrary units. Data presented are the mean of at least three independent analyses.