Table 2.
Identification of copper-binding proteins
| Band n° | Proteina | MW (kDa) | n° peptidesb |
|---|---|---|---|
| 1 | Transketolase 1 | 73.87 | 4 |
| Protein disulfide-isomerase | 58.53 | 1 | |
| 2 | NADP glutamate dehydrogenase 1 | 49.88 | 14 |
| NADP glutamate dehydrogenase 2 | 49.93 | 6 | |
| 3 | Aspartate-semialdehyde dehydrogenase | 40.03 | 3 |
| Cystathionine- γ-lyase | 42.51 | 4 | |
| 4 | Glyceraldehyde-3-phosphate dehydrogenase | 35.83 | 4 |
| 5 | Elongation factor 1-β | 22.67 | 3 |
| 6 | Uncharacterized oxidoreductase YMR226C | 29.19 | 3 |
| 7 | Triose phosphate isomerase | 26.89 | 3 |
| 8 | Cu, Zn-superoxide dismutase | 15.95 | 3 |
| 9 | Peptidyl-prolyl cis-trans isomerase | 17.49 | 2 |
| 40S ribosomal protein S26-A | 13.72 | 1 | |
| 10 | Hypothetical protein YIL051C (or MMF1) | 15.95 | 3 |
| 11 | 60S ribosomal protein L19 | 21.69 | 1 |
| 12 | Hypothetical protein YDR032C | 20.96 | 1 |
| 13 | Eucaryotic translation initiation factor 5A-2 | 17.21 | 1 |
| 14 | Glyceraldehyde-3-phosphate dehydrogenase 2 | 35.93 | 3 |
| Glyceraldehyde-3-phosphate dehydrogenase 3 | 35.83 | 4 | |
| 15 | Peroxiredoxin TSA1 | 21.69 | 1 |
| 16 | Ubiquitin-40S ribosomal protein S31 | 17.43 | 2 |
a ESI-MS indicates that bands 1, 2, 3, 9 and 14 contain more than one protein
b number of peptides analyzed by ESI-MS