Figure 5.

Expression of RAGE is similar between male and female Mts1+ mice. A: Whole lung RAGE gene expression is not affected by either over-expression of RAGE or gender as assessed by qRT-PCR. B: Representative images of whole lung sections (x200 magnification) from female wildtype mice, male wildtype mice, female Mts1+ mice and male Mts1+ mice stained for RAGE. RAGE immunoreactivity is localised to the endothelial layer of pulmonary arteries adjacent to airways and is enhanced in both male and female Mts1+ mice compared to wildtype controls. Red arrows point to pulmonary arteries. Scale bars represent 50 μm. B: Representative images of pulmonary arteries not adjacent to airways (x400 magnification) showing RAGE and α-smooth muscle actin immunoreactivity on adjacent lung sections in male and female Mts1+ mice. RAGE appears to be expressed in the medial and adventitial layers of pulmonary arteries not adjacent to airways. Scale bars represent 20 μm.