Hepatic Patt1 abrogation protects against liver steatosis in male mice. (A) Male Patt1 LKO mice showed less lipid accumulation in liver compared with littermate controls (CTR) at the age of 31 weeks. Representative sections of the liver stained with hematoxylin and eosin are presented. Scale bar, 50 μm. (B) Hepatic Patt1 deficiency attenuated age-associated triglyceride (TG) accumulation in liver of male mice. Liver triglyceride was measured at the indicated ages. n = 8–12/group, **P < 0.01. (C, D) Liver free fatty acid (FFA) level was attenuated in male Patt1 LKO mice at the age of 31 weeks, whereas liver total cholesterol (TC) remained at a similar level. n = 10–12/group, *P < 0.05, **P < 0.01. (E) The ratio of liver weight to body weight was similar between the male LKO mice and their littermate controls at the age of 31 weeks. n = 10–12/group. (F, G) Liver function of male Patt1 LKO mice was not significantly affected by liver-specific Patt1 knockout. Liver function was measured by determining serum AST and ALT levels at the age of 31 weeks. n = 10–12/group.