Fig. 4.

Hepatocytes from Patt1 LKO mice are resistant to palmitic acid–induced lipid accumulation. (A) Hepatocytes from Patt1 LKO mice exhibited less lipid accumulation when challenged with the indicated concentrations of palmitic acid for 18 h. Lipid accumulation was measured by Nile red staining. Scale bar, 20 μm. (B) Quantification of the lipid accumulation in (A) was achieved by determining the red fluorescence of Nile red with a plate reader. **P < 0.01 versus control (CTR) in the same group. (C) Patt1 protein levels in the primary mouse hepatocytes treated with the indicated concentrations of palmitic acid (PA) for 18 h were measured by immunoblot. (D) Hepatocytes from Patt1 LKO mice were resistant to PA-induced upregulation of cellular triglyceride levels. *P < 0.05, **P < 0.01 versus CTR in the same group.