Fig. 8.
Altered expression levels of crucial metabolic regulators and BAT-specific factors in DBKO mice compare with ob/ob mice. A: Bar graphs show mRNA levels of indicated genes normalized for the expression of β-actin in male mice fed HFD. The expression in WT mice was assumed to be 1. B and C: Levels of UCP-1 protein analyzed with Western blot in the BAT (0.5 µg), iWAT (40 µg), and muscle (40 µg) from 12-week-old male ob/ob and DBKO mice fed chow or HFD for 8 weeks. Protein data were normalized for the expression of p38MAPK. D: UCP-1 staining of iWAT by immunohistochemistry. Brown stains indicate UCP-1 protein that appear in smaller adipocytes in iWAT of DBKO mice. Bar, 5 µM. These figures are representative of three different experiments. Values given are means ± SE. *P < 0.05; **P < 0.01 (n = 6 per group).