TABLE 1.
Comparative proteomics between lipid droplet proteins of RHA1 cultivated in NB and MSM
| NB | MSM |
| Band 44 | Band 15 |
| 60 kDa chaperonin GroEL (17) | 60 kDa chaperonin GroEL (13) |
| Chaperone protein (8) | |
| Band 45 | Band 16 |
| 60 kDa chaperonin GroEL (12) | 60 kDa chaperonin GroEL (6) |
| Chaperone protein (11) | Chaperone protein (21) |
| Adenylate cyclase family protein (8) | |
| Probable uroporphiryn-III C-methyltransferase (3) | |
| Band 46 | Band 15–27 |
| IMP dehydrogenase/GMP reductase (7) | IMP dehydrogenase/GMP reductase (10) |
| Pyruvate kinase (3) | Pyruvate kinase (5) |
| FA CoA dehydrogenase (3) | Probable UDP-glucose 4-epimerase (6) |
| Pyruvate kinase (5) | |
| Probable As(2+)-transporting ATPase (5) | |
| Probable linoleoyl-CoA desaturase (4) | |
| AFG1-like ATPase|RHA1_ro06866 (4) | |
| Possible hydrolase (4) | |
| Probable oxidoreductase (4) | |
| NAD(P) transhydrogenase α subunit (3) | |
| Probable glutaryl-CoA dehydrogenase (3) | |
| Acyl CoA dehydrogenase (3) | |
| Aspartate-semialdehyde dehydrogenase (3) | |
| Band 47 | Band 31 |
| Possible phage shock protein (9) | Possible phage shock protein (6) |
| Apolipoprotein A1/A4/E (9) | Sporulation regulator whiA N-terminal (6) |
| Sporulation regulator whiA N-terminal (8) | Methyltransferase domain (4) |
| Diacylglycerol kinase catalytic domain (4) | Glycosyltransferase (7) |
| 30S ribosomal protein S3 (4) | H(+)-transporting two-sector ATPase γ subunit (5) |
| NAD dependent epimerase/dehydratase (3) | Hypothetical protein (5) |
| Probable arginine transport ATPase (3) | FeS assembly ATPase (4) |
| Methyltransferase domain (3) | Ribose-phosphate diphosphokinase (4) |
| Recombinase A (3) | |
| Cell division initiation protein (3) | |
| α/beta hydrolase of unknown function (3) | |
| Hypothetical protein (3) | |
| Band 48 | Band 32 |
| Possible phage shock protein (11) | Possible phage shock protein (18) |
| Apolipoprotein A1/A4/E (5) | Ribose-phosphate diphosphokinase (8) |
| Ribose-phosphate diphosphokinase (4) | Probable short-chain dehydrogenase (5) |
| Glycosyltransferase (4) | Apolipoprotein A1/A4/E (3) |
| FeS assembly ATPase (3) | Probable ABC multidrug resistance transporter (3) |
| Xylose isomerase-like TIM barrel (3) | |
| Acyl-[acyl-carrier-protein]desaturase (3) |
Lipid droplet proteins were extracted from RHA1 cells cultivated in NB and MSM, respectively. After silver staining, the main bands of lipid droplet protein lanes were cut corresponding to the same position and subjected to LC-MS analysis (Fig. 2B). The numbers in parentheses are peptide numbers identified through LC-MS.