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Kinetic characteristics of PS-PLA1 and single amino acid mutants
| Substrate: LPS |
Substrate: PS |
Vmaxratio | |||
| Enzyme | Vmax(pmol/min/ng protein) | apparent Km(μM) | Vmax(pmol/min/ng protein) | apparent Km(μM) | (LPS/PS) |
| PS-PLA1 | 245 | 38 | 113 | 26 | 2.2 |
| A93P | 11.6 | 20 | 49.6 | 24 | 0.23 |
| L94T | 192 | 25 | 73.6 | 21 | 2.6 |
| T96S | 118 | 31 | 62.8 | 16 | 1.9 |
| K97P | 259 | 28 | 129.6 | 11 | 2.0 |
LPS, lysophosphatidylserine; PS, phosphatidylserine.
