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. 2012 Mar;53(3):529–539. doi: 10.1194/jlr.M014688

Fig. 2.

Fig. 2.

Stimulation of the CES1A1 promoter. (A) Concentration-dependent induction of CES1 and BSEP Huh7 cells were treated with Z-guggulsterone (Gugg) at various concentrations (0–20 µM) for 24 h, and then the levels of CES1 and BSEP mRNA were determined as described above. An asterisk indicates statistical significance of a treatment over solvent control, and an alpha indicates statistical significance between two columns linked by a line (P < 0.05). (B) Effect of actinomycin D (Act D) on induction. Huh7 cells were treated with Z-guggulsterone (5 μM) for 24 h in the absence or presence of actinomycin D (1 μM), and the levels of CES1 and BSEP mRNA were determined. An asterisk indicates statistical significance of a treatment over solvent control (P < 0.05). (C) Activation of CES1A1 reporters. Huh7 cells were seeded in 48-well plates. After an overnight incubation, the cells were transfected with a reporter (50 ng) along with 5 ng of the null-Renilla luciferase plasmid. The transfected cells were treated with Z-guggulsterone (10 μM) or the same volume of DMSO for 24 h. Luciferase activities were determined with a dual-luciferase reporter assay. Data in this figure are from three separate experiments.