TABLE 2.
Validation data for the analyzed retinoids
| Analytes | Equation for Calibration Curve | r2 | LLOQ (intraday, interday CV%)a | Middle LOQ (intraday, interday CV%)b |
| atRA | Y = 1.67x − 0.12 | 0.995 | 5.4, 15.0 | 3.6, 6.9 |
| 9-cisRA | Y = 1.43x − 0.18 | 0.996 | 14.3, 19.6 | 2.1, 4.9 |
| 13-cisRA | Y = 1.96x + 0.18 | 0.994 | 7.3, 13.7 | 3.2, 3.3 |
| 4oxo-atRA | Y = 2.41x + 0.55 | 0.982 | 15.8, 17.9 | 4.0, 13.8 |
| 4oxo-13cisRA | Y = 7.64x − 0.42 | 0.997 | 10.8, 18.8 | 6.5, 6.7 |
a
The LLOQs were determined at 0.05 nM RA isomers and 6 nM 4oxo-RA isomers.
b
The LOQs were determined at 7.5 nM RA isomers and 10 nM 4oxo-RA isomers.