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. 2012 Feb 9;7(2):e31317. doi: 10.1371/journal.pone.0031317

Figure 1. Effects of 13-oxo-ODA on PPARα activation determined by luciferase assay and using mouse primary hepatocytes.

Figure 1

(A) Chemical structures of 13-oxo-ODA, 9-oxo-ODA, and CLA. (B) A reporter plasmid (p4xUASg-tk-luc) and an expression vector for a GAL4 PPARα chimeric protein (pM-hPPARα) were transfected into CV1 cells together with an internal control reporter plasmid (pRL-CMV). Twenty-four hours after the transfection, the cells were treated with synthesized CLA or 13-oxo-ODA for 24 h. GW7647 (5 nM), which is a PPARα-specific agonist, was used as a positive control. Luciferase activity was measured using a dual luciferase system. The activity of a vehicle control was set at 100%, and the relative luciferase activities are presented as fold induction with respect to that of the vehicle control. Data are presented as mean ± SEM (n = 4−5). **; p<0.01 versus control. #; p<0.05 compared between indicated groups.