Figure 2. Glutamatergic neurons from different brain areas form synaptic contacts with myotubes in vitro.

Confocal images showing synapses between myotubes and glutamatergic neurons from cortex (A–F), cerebellum (G–L) or hippocampus (M–O) after 9 days of coculture and immunostained. AMPARs (GluR1 subunit) are in green, axonal neurofilaments and terminations are in red (NF, SV2), whereas AChRs are in cyan (α-bungarotoxin). AChRs are diffusely distributed on myotube surface, whereas AMPARs form clusters that are often near to the axonal termination. Scale bars 10 µm.