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. 2012 Feb 9;7(2):e31678. doi: 10.1371/journal.pone.0031678

Figure 2. Kosmotropic anions induce formation of PrPSc-like protease-resistant species.

Figure 2

A–F: RecPrP was incubated with different concentrations of sodium sulfate (Na2SO4, A ), sodium chloride (NaCl, B), tetramethylammonium sulfate (((CH3)4N)2(SO4), C,E) and tetramethylammonium chloride ((CH3)4N(Cl), D,F) as described in Experimental Procedures, followed by Western Blotting (A–D) or silver staining (E , F). Salts concentrations (mM) for the reactions were 0, 100, 200, 300, 400 and 500 for lanes 1, 2, 3, 4, 5 and 6, respectively. Undigested recPrP standard is shown on lane 7 for each figure. The arrows indicate the signal corresponding to proteainse K (PK).