Figure 4. Effects of heterozygous mutant alleles on YFP and CFP fluorescence differ among mutational classes in diploid cells.

(A) The effect of each mutant genotype on YFP fluorescence in haploid cells (X-axis) is compared to the effect of the heterozygous mutant genotype on YFP fluorescence in diploid cells (Y-axis). These diploid cells were heterozygous for the mutant P_TDH3-YFP haploid genome and a reference haploid genome containing P_TDH3-CFP. Black squares indicate cis-regulatory mutants, blue circles indicate trans-acting mutants, green triangles indicate coding mutants, and red crosses indicate CNV mutants. (B) Using the same symbols to represent the four mutational classes as in (A), the effect of each mutant on YFP (X-axis) and CFP (Y-axis) fluorescence in diploid cells is shown. Insets in (A) and (B) show only coding mutants and cover the larger ranges of Z-scores needed to plot all of the mutants in this class.