Skip to main content
. 2012 Feb 9;8(2):e1002524. doi: 10.1371/journal.ppat.1002524

Figure 6. Expression of full-length Bd3459 protein alone, but not a S70A active site variant, causes rounding/lysis of E. coli.

Figure 6

Time-lapse microscopy of E. coli TOP10 cells containing either wild type (WT) or active site variant (S70A) Bd3459 protein being overexpressed from an L-arabinose inducible plasmid by addition of arabinose at 0 hours. Cells were immobilised on a YT-agarose pad (YT) or osmotically stabilised M-medium-agarose pad (M), containing 0.2% (v/v) L-arabinose and development over 6 hours is shown. A = Bd3459 (WT) on YT; B = Bd3459 (S70A) on YT; C = Bd3459 (WT) on M; D = Bd3459 (S70A) on M. Osmotically unsupported E. coli expressing WT Bd3459 lyse and osmotically supported E. coli round up. E. coli expressing the S70A protein variant continues to grow normally. Also see Videos S13, S14, S15, S16.