Figure 5. LecRK-V.5 is localized at the plasma membrane and is expressed at guard cells upon PTI activation.

(A, B and C) Subcellular localization of LecRK-V.5-GFP fusion protein in Arabidopsis mesophyll protoplasts. LecRK-V.5-GFP expression was driven by the cauliflower mosaic virus 35S promoter and transiently expressed in Arabidopsis mesophyll protoplasts. The bright-field image (A), images of the GFP fluorescence (green) only (B), and the overlap image of the GFP (green) and chlorophyll (red) fluorescence (C) are presented. (D) Photo of a control protoplast expressing GFP alone. Bars = 20 µm. (E) The activity of LecRK-V.5 promoter was detected by GUS staining in guard cell 15 min after inoculation with 1×10⁸ cfu.ml⁻¹ Pst DC3000 (Pst) or 1 µM flg22 treatment. Bar represents 20 µm.