Figure 3. Colony morphology and melanized appressoria of the chs deletion mutants.

(A) Colonies of the wild-type strains P131 and S1528 and the chs1-chs7 mutants formed on the complete medium (CM) at 25°C. Photos were taken at 7 days post-inoculation. Bar = 10 mm. (B) Appressorium formation assays with conidia from the same set of the wild-type and mutant strains. Representative images were taken after 24 h incubation on glass coverslips. Germ tubes of the chs7 mutant failed to form appressoria. Bar = 10 µm.