Table 2 . Glycerolipid precursor enzymes and transporters.
| Gene | Ino− or Opi− phenotype | Enzyme | Mol mass (kDa) | Isoelectric point | Molecules per cellb | Locationc | Transmembrane domains | Phosphorylation sitesd |
|---|---|---|---|---|---|---|---|---|
| ACC1 (ABP2,FAS3,MTR7)a | — | Acetyl CoA carboxylase | 250.4 | 6.22 | 20,200 | Cytoplasm | None | Several |
| HFA1 | — | Acetyl CoA carboxylase | 241.8 | 8.05 | 396 | Mitochondria | None | Few |
| FAS1a | — | Fatty acid synthase (β subunit) | 228.7 | 5.79 | 91,800 | Cytoplasm | None | Several |
| FAS2a | — | Fatty acid synthase (α subunit) | 206.9 | 5.21 | 17,000 | Cytoplasm | None | Several |
| ETR1(MRF’) | Ino− | 2-Enoyl thioester reductase | 42 | 9.78 | 1560 | Mitochondria | None | None |
| HTD2 (RMD12) | — | 3-Hydroxyacyl-thioester dehydratase | 33 | 9 | 799 | Mitochondria | None | None |
| MCT1 | — | Malonyl-CoA:ACP transferase | 40.7 | 6.9 | 1360 | Mitochondria | None | None |
| OAR1 | — | 3-Oxoacyl-ACP reductase | 31.2 | 9.3 | 1760 | Mitochondria | None | None |
| ACP1 | — | Acyl carrier protein | 13.9 | 4.64 | 60,500 | Mitochondria | None | Few |
| PPT2 | — | Phosphopantetheine:protein transferase | 19.9 | 8.46 | 486 | Mitochondria | None | None |
| CEM1 | — | β-keto-acyl synthase | 47.6 | 8.26 | 1660 | Mitochondria | None | None |
| FAA1 | — | Fatty acyl CoA synthetase | 77.8 | 7.58 | 7470 | ER, lipid droplets | None | None |
| FAA2 (FAM1) | — | Fatty acyl CoA synthetase | 83.4 | 7.7 | 358 | Peroxisomes | None | None |
| FAA3 | — | Fatty acyl CoA synthetase | 77.9 | 9.7 | 6440 | Plasma membrane | None | None |
| FAA4 | — | Fatty acyl CoA synthetase | 77.2 | 6.52 | 31,200 | ER, lipid droplets | None | None |
| FAT1 | — | Fatty acid transporter and fatty acyl CoA synthetase | 77.1 | 8.47 | 16,900 | ER, lipid droplets | 3 | None |
| OLE1 (MDM2) | — | Δ9 Fatty acid desaturase | 58.4 | 9.71 | ND | ER | 4 | None |
| ELO1 | — | FA elongase, condensing enzyme | 36.2 | 10.2 | 937 | ER | 5 | None |
| FEN1 (ELO2) | — | FA elongase, condensing enzyme | 40.0 | 10.35 | 3510 | ER | 7 | Several |
| SUR4 (ELO3) | — | FA elongase, condensing enzyme | 39.5 | 10.13 | ND | ER | 6 | None |
| IFA38 (YBR159w) | — | β-keto acyl-CoA reductase | 38.7 | 10.28 | 41900 | ER | 1 | None |
| PHS1 | — | 3-Hydroxy acyl-CoA dehydratase | 24.5 | 10.84 | ND | ER | 6 | None |
| TSC13 | — | Enoyl-CoA reductase | 36.8 | 10.38 | 23600 | ER | 4 | None |
| INO1a (APR1) | Ino− | Inositol 3-P synthase | 59.6 | 5.77 | ND | Cytoplasm | None | None |
| INM1 | — | Inositol 3-P phosphatase | 32.8 | 5 | 2440 | Cytoplasm, nucleus | None | None |
| URA7 | Ino− | CTP synthetase | 64.7 | 5.93 | 57,600 | Cytoplasm | None | Several |
| URA8 | — | CTP synthetase | 63 | 6.02 | 5370 | Cytoplasm | None | None |
| ITR1a | Opi− | Inositol transporter | 63.5 | 6.51 | ND | Plasma membrane | 12 | Several |
| ITR2 (HRB612) | — | Inositol transporter | 66.7 | 8.25 | 468 | Plasma membrane | 12 | None |
| HNM1 (CTR1)a | — | Choline transporter | 62 | 6.83 | ND | Plasma membrane | 12 | Few |
| GIT1 | — | GroPIns/GroPCho transporter | 57.3 | 8.64 | ND | Plasma membrane | 11 | None |
Much of the information in the table may be found in the Saccharomyces Genome Database. Ino−, inositol auxotrophy; Opi−, inositol excretion; ND, not determined.
a
Genes containing the UASINO element and are regulated by the Ino2-Ino4-Opi1 circuit, The names in parentheses are aliases.