Figure 5 .

CK1α^8B12 clones induce ectopic Wg target gene expression. (A) Adult eyes containing CK1α^8B12 clones display cuticle protrusions in the retina (inset in A’). (B and C) CK1α^8B12 (B) or sgg^8C18 (C) mutant clones in the eye disc marked by the absence of arm-lacZ staining (blue) are overgrown, lack Elav staining (brown), and have smooth boundaries. (D–G) Eye discs stained for ds-lacZ (D,E) or Omb (F and G). ds-lacZ (D) and Omb (F) are normally expressed at the anterior lateral margins of the eye disc (solid arrowheads). Omb also marks retinal glial cells (open arrowhead). (E–G) GFP (green)-negative CK1α^8B12 clones ectopically express ds-lacZ (red in E) and Omb (red in G). (H and I) Adult wing blades of wild type (H) or flies carrying CK1α^8B12 clones (I). CK1α^8B12 clones differentiate ectopic bristles (inset in I). (J–L) Third instar wing discs carrying GFP-negative CK1α^8B12 clones. CK1α^8B12 clones in the wing pouch (arrowheads) ectopically express high levels of Sens (red in J) and Dll-lacZ (red in K), but do not misexpress vgBE-lacZ (red in L). Cells outside the wing pouch fail to induce ectopic expression (chevrons in J and K). Normal expression of these genes at and/or adjacent to the D/V boundary of the wing pouch is shown by open arrowheads.