Table 1.
Antibody provenances
| Antibodies/antisera | Source | Catalog no. | Controls |
References | ||||
|---|---|---|---|---|---|---|---|---|
| Tested in KO? | Immuno | IP | Western | Pre-absorp | ||||
| Structural proteins | ||||||||
| Contactin (ms) | NeuroMab | 75-038 | X (ON) | X | Datasheet | |||
| Caspr1 (ms) | Rasband/NeuroMab | Gift, 75-001 | X (w) | X (Br) | X | Rasband and Trimmer (2001); datasheet | ||
| Caspr2 (ms) | NeuroMab | 75-075 | X (w) | X (Br) | X | Datasheet | ||
| Caspr2 (rb) | US Biologicals | C2089-70 | X | Datasheet | ||||
| Caspr3 (rb) | Peles | Gift | X (Br) | X | Spiegel et al. (2002) | |||
| Caspr4 (rb) | Peles | Gift | X (Br) | X | Spiegel et al. (2002) | |||
| AnkyrinB (rb)/(ms) | Bennett/NeuroMab | Gift, 75-144; 75-145 | X (w, if) | X (Br) | X | Datasheet; Scotland et al. (1998) | ||
| AnkyrinG (rb)/(ms) | Bennett/NeuroMab | Gift, 75-146; 75-147 | X (Br, ON) | Datasheet; Kordeli et al. (1995) | ||||
| βIV Spectrin (rb) | Rasband | Gift | X (if) | X (Br, PNS) | X | X | Lacas-Gervais et al. (2004) | |
| Pan-neurofascin | Rasband | Gift | X (ON) | X | X | Schafer et al. (2004) | ||
| Neurofascin 186 (ms) | Gow/Rasband | Gift | X (SN) | X | Schafer et al. (2006) | |||
| Ezrin (ms) | Zymed/Invitrogen | 35-7300 | X (cells) | Datasheet | ||||
| Pan-dystrophin (H4) (rb) | Roux/Mornet | Gift | X (Rt) | X | Rivier et al. (1999) | |||
| Tenascin-C (rb) | Chemicon/Millipore | AB19013 | X (H, V) | X | Datasheet; Huss et al. (2010) | |||
| Tenascin (gt) | Santa Cruz | SC 9871 | Datasheet | |||||
| Neurofilament 165 (ms) | DSHB | 2H3 | X (SC) | Dodd et al. (1988) | ||||
| NaV channels | ||||||||
| NaV1.1 (ms) | NeuroMab | 75-023 | X (Br) | X | Datasheet | |||
| NaV 1.2 (ms) | NeuroMab | 75-024 | X (Br) | X | Datasheet | |||
| Nav1.3 (rb) | Alomone | ASC-004 | X (DRG) | X | X (w) | Datasheet | ||
| NaV 1.5 (rb) | Chemicon/Millipore | AB5493 | X (V, H) | X | X (w) | Datasheet; Wooltorton et al. (2007) | ||
| NaV 1.6 (rb) | Alomone | ASC-009 | X (SN, Br, DRG) | X | X (w, if) | Black et al. (2002) | ||
| NaV 1.6 (rb)/(ms) | Rasband/NeuroMab | Gift, 75-026 | X (if) | X (ON, Br) | Rasband et al. (2003); datasheet | |||
| Pan NaV (rb) | Sigma | S6936 | X | Datasheet | ||||
| Pan NaV (ms) | Sigma | S8809 | X (ON, SN, Br) | X | Datasheet; Rasband et al. (1999) | |||
| Pan Nav (rb) | Alomone | ASC-003 | X (Br) | X | X (w) | Datasheet | ||
| Pan NaV (ms) | Levinson | Gift | Rasband et al. (1999) | |||||
| KV channel subunits | ||||||||
| KV 1.1 (ms) | NeuroMab | 75-007 | X (w) | X (Br) | X | Datasheet | ||
| KV 1.2 (ms) | NeuroMab | 75-008 | X (w) | X (Br) | X | Datasheet | ||
| KCNQ2-N1 (rb)*urea | Cooper | Gift | X (Br) | X | X | X (w, if) | Cooper et al. (2001); Devaux et al. (2004) | |
| KCNQ3-N1 (gp) long | Cooper | Gift | X (Br) | X | Devaux et al. (2004) | |||
| KCNQ3-C1 (rb) | Cooper | Gift | X (Br) | X | X | X (w) | Cooper et al. (2000) | |
| KCNQ4 (rb) | Jentsch | Gift | X (w) | X | X | Kharkovets et al., (2000, 2006) | ||
| KCNQ5 (rb) | Jentsch | Gift | X (cells) | X | Tzingounis et al. (2010) | |||
| KCNQ5 (rb) | Villarroel | Gift | Yus-Nájera et al. (2003) | |||||
| ERG1 (rb) | Chemicon/Millipore | AB5222 | X (V) | X | X (w) | Datasheet; Hurley et al. (2006) | ||
| ERG2 (rb) | Alomone | APC-114 | X (Br) | X | X (w) | Datasheet | ||
| ERG3 (rb) | Alomone | APC-112 | X (Br) | X | X (w) | Datasheet | ||
| MiRP1 (rb) | Chemicon/Millipore | AB5845 | X | X (w) | Datasheet | |||
| SK2 (rb) | Chemicon/Millipore | AB5356 | X (Br) | X | Datasheet | |||
| Myelin basic protein | ||||||||
| MBP (ms) | Covance | SMI-99P | X (Br) | X | Datasheet | |||
| Calretinin | ||||||||
| Calretinin (gt) | Chemicon/Millipore | AB1550 | X (Br, Rt) | X | X (w) | Datasheet; Desai et al. (2005) | ||
| Calretinin (rb) | Chemicon/Millipore | AB5054 | X (Br) | X | X (w) | Datasheet | ||
| Calretinin (ms) | Chemicon/Millipore | MAB1568 | X (Br) | X | Datasheet | |||
KO, Knock-out; Immuno, immunohistochemistry; Western, Western blot; IP, immunoprecipitation; Pre-absorp, preabsorption; ms, mouse; rb, rabbit; gt, goat; gp, guinea pig; Br, brain; ON, optic nerve; SN, sciatic nerve; V, vestibular tissue; H, heart; Rt, retina; PNS, peripheral nervous system; DRG, dorsal root ganglia; SC, spinal cord; w, Western blot; if, immunofluorescence; ip, immunoperoxidase.