CHRDL1 Is Expressed in the Developing Anterior Segment and Cornea
(A) RT-PCR of human eye tissues showing CHRDL1 expression in the developing cornea from week 7 of gestation continuing into adulthood. CHRDL1 is also expressed in the developing lens and retina and in adult trabecular meshwork. PAX6 (MIM 607108) was used as a control.
(B–H) In situ hybridization of CHRDL1 in the human eye at week 8 of gestation (Carnegie fetal stage F1; sagittal sections) showing expression in the neural retina (NR), and the anterior periocular mesenchyme (arrowheads) of the developing anterior segment angle and cornea. The following abbreviations are used: A, anterior segment angle; C, cornea; L, Lens; En, corneal endothelium; St corneal stroma; Ep, corneal epithelium; LEp, lens epithelium. Sense probe control is shown in (D). The scale bars represent 200 μm (A and D), 100 μm (C), 50 μm (E–G), and 20 μm (H).