Fig. 2. T24 Bladder Tumor Cells Internalize Antibody-crosslinked His₆-FAP:trisNTA-AlexaFluor647 into Rab5^Q79L-GFP Labeled Compartments.

A. Cartoon representing the antibody-induced FAP microaggregation strategy (see text for details). B. Scheme depicting the fusogenic effects of Rab5 dominant positive (Q79L) on the endosomal compartments. C. T24 cells expressing Rab5^Q79L-GFP were exposed to 10μM His₆-FAP for 1h at 37°C. After washing, the cells were incubated with Ni²⁺:trisNTA-AlexaFluor647 and either α-HA (upper row) or α-FAP (lower row) polyclonal antibodies for 45min at 10°C. After washing with cold PBS, the cells were incubated 3h at 37°C and fixed. Arrows point to some areas of colocalization. Scale bar: 20microns