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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Neurobiol Dis. 2011 Dec 11;45(3):902–912. doi: 10.1016/j.nbd.2011.12.007

Figure 1. EID1 is highly enriched in neurons and much less in astrocytes.

Figure 1

A. Double staining of the Cornu Ammonis 3 (CA3) region of mouse hippocampus with anti-EID1 and anti-NeuN antibodies demonstrating that EID1 is expressed in neurons. B. Double staining of similar brain region (CA3) with anti-EID1 and anti-GFAP antibodies showing that there is no or less expression of EID1 in GFAP-positive cells. Rhodomine-conjugated anti-rabbit IgG (for EID1) and FITC-conjugated anti-mouse IgG (for NeuN and GFAP) were used to detect the specific immunostaining. Scale bar = 100 µM. C. Changes in mRNA levels of EID1 during RA-induced differentiation of NT2 cells were determined by qRT-PCR. The samples were measured against the cDNA of undifferentiated NT2 cells as a control, set at 100%. Percentage of each sample was calculated by 100× 2−ΔCt, where ΔCt is the cycle number difference between test sample and the control sample. undiff – undifferentiated NT2 cells (control), NT2A – NT2 astrocytes, NT2N – NT2 neurons. The experiments were performed in triplicate. D. Western blotting analysis of the three cell types shown in C indicating that EID1 protein is highly enriched in neuronal cells. β-actin was used as a loading control.