Figure 2. Subcellular localization of EID1 protein in control and AD brains.
The middle temporal gyrus of control and AD human brains was stained with anti-EID1 and anti-NeuN (A), or anti-EID1 and anti-GFAP (B) antibodies. Rhodomine-conjugated anti-rabbit IgG (for EID1) and FITC-conjugated anti-mouse IgG (for NeuN and GFAP) were used to detect the specific immunostaining. Scale bar = 30 µM. C. The middle temporal gyrus of control and AD human brains was stained with anti-EID1 and anti-NeuN antibodies. The nuclei were counterstained with DAPI. Scale bar = 10 µM. D. Western blotting analysis of fractionated EID1 protein from control and AD brains illustrating the increase of nuclear localization of this protein in AD brain.