Skip to main content
. Author manuscript; available in PMC: 2013 Jan 12.
Published in final edited form as: Oncogene. 2011 Nov 7;31(28):3370–3380. doi: 10.1038/onc.2011.496

Figure 1. Constitutive Hh signaling in breast cancer cell lines enhances their malignant attributes.

Figure 1

A) Cell lysates and concentrated (10X) conditioned media from each experimental group for stable SHH-expressors of MDA-MB-231 cells were assessed by western blot analyses. Activated Hh signaling significantly enhances B) Cell proliferation (^p < 0.001, for both, SHH cl. 3 and Mixed Pool relative to Vector control cells), C) Contact independent growth (^p = 0.03 for SHH cl. 3, and p > 0.05 for Mixed Pool relative to vector controls), D) Anchorage independent growth (^p < 0.0001 for all SHH-expressing groups relative to vector control cells), E) Non-chemotactic cell movement (^p < 0.0001 for all SHH-expressing groups relative to vector control cells) and, F) Invasion through Matrigel (^p < 0.0001 for all SHH-expressing groups relative to vector control cells).