Fig. 6.

a–f Fluorescence images showing the effect of TNF-α incubation on HMEC cells stained for F-actin fibers (green) and nucleic acids (blue). Visible scale bars—\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$100\;\mu\text{m}$\end{document}. In the absence of cytokine, cells create brick-like pattern with actin filaments placed tangentially around the nucleus. Short incubation times brings the elevation of F-actin at cell edges (arrows 2 and 4), while parallel arrangement of fibers along cell main axis is also observed (arrows 1 and 3). Longer incubation time brings reorganization of the cytoskeleton, which manifests in shortening of parallel fiber groups (arrows 5, 7, and 8)