Table 1.
Morphometric data on macrostome (upper line) and microstome (lower line) Leptopharynx bromelicola n. sp.
| Characteristicsa | Mean | M | SD | SE | CV | Min | Max | n | % Increaseb |
|---|---|---|---|---|---|---|---|---|---|
| Body, length in protargol preparations (μm) | 49.7 | 50.0 | 4.3 | 1.0 | 8.6 | 41.0 | 58.0 | 20 | 46.0 |
| 34.0 | 34.0 | 3.4 | 0.7 | 10.0 | 29.0 | 39.0 | 21 | ||
| Body, width in protargol preparations (μm) | 39.0 | 39.0 | 4.2 | 0.9 | 10.8 | 30.0 | 46.0 | 20 | 57.3 |
| 24.8 | 25.0 | 2.6 | 0.6 | 10.4 | 20.0 | 30.0 | 21 | ||
| Body, length in Chatton–Lwoff silver nitrate preparations (μm) | 54.5 | 53.0 | 5.2 | 1.2 | 9.6 | 47.0 | 65.0 | 20 | 50.6 |
| 36.2 | 37.0 | 4.1 | 0.9 | 11.3 | 30.0 | 45.0 | 21 | ||
| Body, width in Chatton–Lwoff silver nitrate preparations (μm) | 32.4 | 32.5 | 4.0 | 0.9 | 12.3 | 26.0 | 40.0 | 20 | 45.9 |
| 22.2 | 22.0 | 3.5 | 0.8 | 15.9 | 18.0 | 32.0 | 21 | ||
| Body, length in SEM macrostomes (μm) | 43.1 | 44.0 | 4.6 | 1.1 | 10.6 | 31.0 | 50.0 | 17 | — |
| Body, width in SEM macrostomes (μm) | 28.9 | 30.0 | 2.7 | 0.7 | 9.4 | 24.0 | 34.0 | 17 | — |
| Body length: width, ratio in the SEM | 1.5 | 1.5 | 0.1 | 0.1 | 8.8 | 1.3 | 1.7 | 17 | — |
| Body length: width, ratio in protargol preparations | 1.3 | 1.2 | 0.1 | 0.1 | 11.3 | 1.0 | 1.5 | 20 | −7 |
| 1.4 | 1.3 | 0.2 | 0.1 | 16.9 | 1.1 | 1.9 | 21 | ||
| Body length: width, ratio in Chatton–Lwoff silver nitrate preparations | 2.1 | 2.0 | 0.3 | 0.1 | 15.0 | 1.5 | 3.0 | 20 | 23.5 |
| 1.7 | 1.7 | 0.1 | 0.1 | 8.2 | 1.3 | 1.9 | 21 | ||
| Anterior body end to adoral membranelles, distance (μm) | 8.0 | 8.0 | 1.2 | 0.3 | 15.0 | 6.0 | 10.0 | 20 | 14.3 |
| 7.0 | 7.0 | 1.0 | 0.2 | 14.3 | 5.0 | 9.0 | 21 | ||
| Anterior body end to macronucleus, distance (μm) | 18.2 | 18.5 | 2.6 | 0.6 | 14.5 | 13.0 | 22.0 | 20 | 32.8 |
| 13.7 | 14.0 | 2.7 | 0.6 | 19.5 | 9.0 | 19.0 | 21 | ||
| Anterior body end to excretory pore of contractile vacuole (μm) | 28.5 | 29.0 | 2.6 | 0.6 | 9.3 | 23.0 | 33.0 | 20 | 62.9 |
| 17.5 | 18.0 | 2.0 | 0.4 | 11.4 | 14.0 | 21.0 | 21 | ||
| Macronucleus, length (μm) | 9.0 | 9.0 | 0.7 | 0.2 | 7.7 | 8.0 | 10.0 | 20 | 26.8 |
| 7.1 | 7.0 | 0.9 | 0.2 | 11.9 | 6.0 | 10.0 | 21 | ||
| Macronucleus, width (μm) | 8.4 | 9.0 | 0.8 | 0.2 | 9.0 | 7.0 | 9.0 | 20 | 20.0 |
| 7.0 | 7.0 | 0.6 | 0.1 | 9.0 | 6.0 | 8.0 | 21 | ||
| Micronucleus, diameter (μm) | 2.7 | 2.6 | — | — | — | 2.0 | 3.0 | 20 | 12.5 |
| 2.4 | 2.3 | — | — | — | 2.0 | 2.8 | 21 | ||
| Oral basket at distal end, long axis (μm), protargol impregnation | 13.1 | 13.0 | 1.1 | 0.2 | 8.0 | 11.0 | 15.0 | 20 | 77.0 |
| 7.4 | 7.0 | 0.9 | 0.2 | 12.5 | 5.0 | 9.0 | 21 | ||
| Oral basket at distal end, short axis (μm), protargol impregnation | 8.3 | 8.0 | 1.0 | 0.2 | 12.4 | 6.0 | 10.0 | 20 | 69.4 |
| 4.9 | 5.0 | 0.7 | 0.2 | 14.3 | 3.0 | 6.0 | 21 | ||
| Oral basket at distal end, short axis (μm), Chatton–Lwoff preparation | 2.1 About 1 μm |
2.0 | 0.3 | 0.1 | 15.0 | 1.5 | 3.0 | 20 | — |
| Oral basket of macrostomes at distal end, long axis in SEM micrographs (μm) | 10.6 | 11.0 | 1.2 | 0.3 | 11.7 | 8.0 | 13.0 | 23 | — |
| Oral basket of macrostomes at distal end, short axis in SEM micrographs (μm) | 2.2 | 2.2 | 0.5 | 0.1 | 23.5 | 1.4 | 3.0 | 17 | — |
| Oral basket, number of rods (nasse kinetosomes) | 47.9 | 48.5 | 3.1 | 0.7 | 6.5 | 42.0 | 52.0 | 20 | 49.2 |
| 32.1 | 32.0 | 2.8 | 0.6 | 8.5 | 25.0 | 36.0 | 21 | ||
| Somatic kineties, number | 9.0 | 9.0 | 0.0 | 0.0 | 0.0 | 9.0 | 9.0 | 20 | 0.0 |
| 9.0 | 9.0 | 0.0 | 0.0 | 0.0 | 9.0 | 9.0 | 21 | ||
| Somatic kinety 1, number of dikinetids | 17.4 | 17.5 | 1.4 | 0.3 | 7.8 | 14.0 | 20.0 | 20 | 55.4 |
| 11.2 | 11.0 | 1.5 | 0.3 | 13.1 | 8.0 | 13.0 | 21 | ||
| Somatic kinety 1, number of monokinetids | 2.3 | 2.0 | 1.2 | 0.3 | 53.7 | 1.0 | 6.0 | 20 | 27.8 |
| 1.8 | 2.0 | 0.7 | 0.2 | 41.4 | 0.0 | 3.0 | 21 | ||
| Somatic kinety 2, number of dikinetids | 14.1 | 14.0 | 1.8 | 0.4 | 12.5 | 11.0 | 17.0 | 20 | 131.1 |
| 6.1 | 6.0 | 1.0 | 0.2 | 16.3 | 4.0 | 8.0 | 21 | ||
| Somatic kinety 2, number of monokinetids | 25.6 | 26.5 | 2.9 | 0.6 | 11.3 | 21.0 | 31.0 | 20 | 37.6 |
| 18.6 | 18.0 | 3.4 | 0.7 | 18.4 | 14.0 | 26.0 | 21 | ||
| Somatic kinety 3, number of monokinetids (does not have dikinetids) | 45.5 | 45.0 | 4.1 | 0.9 | 8.9 | 34.0 | 51.0 | 20 | 91.2 |
| 23.8 | 22.0 | 4.0 | 0.9 | 16.8 | 18.0 | 30.0 | 21 | ||
| Somatic kinety 4, number of monokinetids (does not have dikinetids) | 47.2 | 47.0 | 4.3 | 1.0 | 9.1 | 36.0 | 55.0 | 20 | 67.4 |
| 28.2 | 28.0 | 3.2 | 0.7 | 11.4 | 23.0 | 35.0 | 21 | ||
| Somatic kinety 5, number of monokinetids (does not have dikinetids) | 34.5 | 34.0 | 5.2 | 1.2 | 15.1 | 18.0 | 42.0 | 20 | 128.5 |
| 15.1 | 15.0 | 3.1 | 0.7 | 20.6 | 12.0 | 21.0 | 21 | ||
| Somatic kinety 6, number of monokinetids (does not have dikinetids) | 18.6 | 19.0 | 2.7 | 0.6 | 14.7 | 12.0 | 24.0 | 20 | 70.6 |
| 10.9 | 10.0 | 1.7 | 0.4 | 15.5 | 9.0 | 15.0 | 21 | ||
| Somatic kinety 7, number of monokinetids (does not have dikinetids) | 9.1 | 9.0 | 1.0 | 0.2 | 11.0 | 8.0 | 11.0 | 20 | 13.8 |
| 8.0 | 8.0 | 1.0 | 0.2 | 13.1 | 6.0 | 10.0 | 21 | ||
| Somatic kinety 8, number of monokinetids (does not have dikinetids) | 4.0 | 4.0 | 0.5 | 0.1 | 11.5 | 3.0 | 5.0 | 20 | 5.3 |
| 3.8 | 4.0 | 0.5 | 0.1 | 14.3 | 3.0 | 5.0 | 21 | ||
| Somatic kinety 9, number of dikinetids | 9.2 | 9.0 | 2.4 | 0.5 | 26.4 | 4.0 | 15.0 | 20 | 283.3 |
| 2.4 | 2.0 | 0.5 | 0.1 | 20.9 | 2.0 | 3.0 | 21 | ||
| Somatic kinety 9, number of monokinetids | 14.0 | 14.0 | 3.5 | 0.8 | 25.4 | 7.0 | 23.0 | 20 | 11.0 |
| 12.7 | 13.0 | 2.5 | 0.6 | 20.0 | 8.0 | 18.0 | 21 | ||
| Preoral ciliary rows, number | 4.0 | 4.0 | 0.0 | 0.0 | 0.0 | 4.0 | 4.0 | 20 | 0 |
| 4.0 | 4.0 | 0.0 | 0.0 | 0.0 | 4.0 | 4.0 | 21 | ||
| Preoral kinety 1, number of dikinetids | 2.1 | 2.0 | — | — | — | 2.0 | 3.0 | 20 | 5.0 |
| 2.0 | 2.0 | 0.0 | 0.0 | 0.0 | 2.0 | 2.0 | 21 | ||
| Preoral kinety 1, number of monokinetids | 0.2 | — | — | — | — | 0.0 | 1.0 | 20 | — |
| 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 21 | ||
| Preoral kinety 2, number of dikinetids | 4.0 | 4.0 | 0.0 | 0.0 | 0.0 | 4.0 | 4.0 | 20 | 29.0 |
| 3.1 | 3.0 | — | — | — | 3.0 | 4.0 | 21 | ||
| Preoral kinety 2, number of monokinetids | 1.9 | 2.0 | 0.6 | 0.1 | 31.7 | 1.0 | 3.0 | 20 | 58.3 |
| 1.2 | 1.0 | — | — | — | 1.0 | 2.0 | 21 | ||
| Preoral kinety 3, number of dikinetids | 6.1 | 6.0 | 0.4 | 0.1 | 7.3 | 5.0 | 7.0 | 20 | 45.2 |
| 4.2 | 4.0 | — | — | — | 4.0 | 5.0 | 21 | ||
| Preoral kinety 3, number of monokinetids | 1.8 | 2.0 | 0.6 | 0.1 | 34.2 | 1.0 | 3.0 | 20 | 5.9 |
| 1.7 | 2.0 | 0.8 | 0.2 | 47.7 | 1.0 | 3.0 | 21 | ||
| Preoral kinety 4, number of dikinetids | 9.1 | 9.0 | 1.0 | 0.2 | 10.6 | 8.0 | 11.0 | 20 | 59.6 |
| 5.7 | 6.0 | 0.6 | 0.1 | 10.2 | 5.0 | 7.0 | 21 | ||
| Preoral kinety 4, number of monokinetids | 5.1 | 5.0 | 1.2 | 0.3 | 23.7 | 3.0 | 8.0 | 20 | 27.5 |
| 4.0 | 4.0 | 1.0 | 0.2 | 25.0 | 2.0 | 5.0 | 21 | ||
| Oral anlage, number of dikinetids | 5.5 | 6.0 | 0.7 | 0.2 | 12.6 | 4.0 | 6.0 | 20 | 175 |
| 2.0 | 2.0 | — | — | — | 2.0 | 3.0 | 21 | ||
| Oral anlage, number of monokinetids | 0.7 | 1.0 | — | — | — | 0.0 | 2.0 | 20 | −30 |
| 1.0 | 1.0 | — | — | — | 0.0 | 1.0 | 21 | ||
| Adoral membranelle 1, number of basal bodies | 3.6 | 4.0 | 0.8 | 0.2 | 21.4 | 2.0 | 4.0 | 20 | −5 |
| 3.8 | 4.0 | 0.5 | 0.1 | 13.4 | 2.0 | 4.0 | 21 | ||
| Adoral membranelle 2, number of basal bodies | 17.1 | 18.0 | 2.0 | 0.4 | 11.5 | 15.0 | 21.0 | 20 | 28.6 |
| 13.3 | 12.0 | 2.2 | 0.5 | 16.5 | 9.0 | 18.0 | 21 | ||
| Adoral membranelle 3, number of basal bodies | 20.4 | 21.0 | 2.1 | 0.5 | 10.2 | 18.0 | 24.0 | 20 | 29.9 |
| 15.7 | 15.0 | 1.9 | 0.4 | 11.9 | 12.0 | 21.0 | 21 | ||
| Resting cysts from macrostomes, length (μm) | 36.3 | 36.0 | 2.4 | 0.8 | 6.6 | 32.0 | 40.0 | 9 | 26.5 |
| Resting cysts from macrostomes, width (μm) | 25.2 | 26.0 | 2.0 | 0.7 | 7.9 | 21.0 | 28.0 | 9 | 43.2 |
| Resting cysts from microstomes, length (μm) | 28.7 | 28.0 | 2.2 | 0.7 | 7.8 | 27.0 | 31.0 | 11 | |
| Resting cysts from microstomes, width (μm) | 17.6 | 17.0 | 1.3 | 0.4 | 7.3 | 16.0 | 20.0 | 11 |
a
Data based, if not mentioned otherwise, on protargol-impregnated, randomly selected specimens from a single culture. See species description, for distinguishing microstomes from macrostomes.
b
% Increase, the increase in the mean value for macrostomes relative to microstomes.
c
CV, coefficient of variation in %; mean, arithmetic mean; M, median; Max, maximum; Min, minimum; n, number of specimens investigated; SD, standard deviation; SE, standard error of mean; SEM, scanning electron microscopy.