Table 1.
Primer details for quantitative PCR
| Primer | Sequence 5′–3′ | Annealing temp (°C) | |
|---|---|---|---|
| Angiopoietin 1 | F | GGGTCACACTGGGACAGCAGG | 58 |
| R | TGGGCCACAGGCATCAAACCA | ||
| ATF41 | F | AGATGACCTGGAAACCATGC | 52 |
| R | AGGGGGAAGAGGTTGAAAGA | ||
| ATF61 | F | AACCAGTCCTTGCTGTTGCT | 52 |
| R | CTTCTTCTTGCGGGACTGAC | ||
| Bax1 | F | CAGGATGCATCCACCAAGAAGC | 56 |
| R | TTGAAGTTGCCGTCGGAAAACATT | ||
| Beta actin2 | F | TGTGCGTGACATCAAGGAGAA | 55 |
| R | CGCAGTGGCCATCTCCTG | ||
| BMP 7 | F | GCCACTTCCTCACCGACGCC | 58 |
| R | CAGCTGCAGTCACGGCCTCC | ||
| CD681 | F | GTCCTGCTACCACCACCAGT | 52 |
| R | GCTGGGAACCATTACTCCAA | ||
| Cyclophilin | F | CATACAGGTCCTGGCATCTTGTC | 56 |
| R | TGCCATCCAACCACTCAGTCT | ||
| DDIT31 | F | AGGACCACCAGAGGTCACAC | 56 |
| R | TGCCACTTTCCTTTCGTTTT | ||
| bFGF | F | CTTTCCCGCCCGGCCACTTT | 58 |
| R | GTCGCTCTTCTCGCGGACCC | ||
| GAPDH2 | F | TCCGTTGTGGATCTGACCTG | 55 |
| R | TGCTTCACCACCTTCTTGATCTC | ||
| GRP781 | F | TGAAACTGTGGGAGGTGTCA | 52 |
| R | TCGAAAGTTCCCAGAAGGTG | ||
| iNOS | F | TGATGCAGAAGGCCATGTCA | 52 |
| R | TCTCCCTGTCTCTGTTGCAAAG | ||
| MCP-1 | F | CAAGACCATCCTGGGCAAA | 52 |
| R | GTCCTGGACCCATTTCAGGTT | ||
| PDGF | F | CAAGACGCGCACGGAGGTGT | 58 |
| R | GCGGTTGTTGCAGCAGCCAG | ||
| SPARC | F | CGAGGTACCCGTGGGAGCCA | 58 |
| R | CAAACTCGCCGATGGGGGCA | ||
| TGFβ1 | F | GGAGTTGTGCGGCAGTGGCT | 58 |
| R | AAGGGCCGGTTCATGCCGTG | ||
| Thrombospondin | F | TGCTGTGCGGGCGGAGAAAG | 58 |
| R | TGCCCGCCTTGCCATTGGAG | ||
| Tie-2 | F | CAGCAGACCTCGGAGGCAGGA | 58 |
| R | TGCCCTCTTCAGCTGCAGCATG | ||
| VEGFA3 | F | GGATGTCTACCAGCGCAGC | 56 |
| R | TCTGGGTACTCCTGGAAGATGTC | ||
| VEGFR13 | F | TGGATTTCAGGTGAGCTTGGA | 52 |
| R | TCACCGTGCAAGACAGCTTC | ||
| VEGFR23 | F | CTTCCAGTGGGCTGATGACC | 52 |
| R | GCAACAAACGGCTTTTCATGT |
All primers designed using NCBI Primer-BLAST; ATF, activating transcription factor; BMP 7, bone morphogenic protein 7; DDIT3, DNA damage inducible transcript 3; bFGF, basic fibroblast growth factor; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; iNOS, inducible nitric oxide synthase; GRP78, 78 kDa glucose-regulated protein; MCP-1, monocyte chemotactic protein 1; PDGF, platelet-derived growth factor; SPARC, secreted protein, acidic, cysteine-rich; TGFβ-1, transforming growth factor β-1; VEGF, vascular endothelial growth factor.