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Plasma levels (mean ± SEM) of (A) ACTH and (B) corticosterone for each euthanasia group were evaluated. The mice that received midazolam displayed a significant (*, P < 0.05) increase in corticosterone but not ACTH; this result was suggestive of a hypoxic ACTH-independent activation of adrenal steroidogenesis. This finding was consistent with the increased time to death following unconsciousness in this group.
