Figure 1.

Effect of 13-desmethyl C spirolide on the viability of various cell types grown in culture. Cells were incubated for 24 h in the presence of 100 nM 13-desmethyl C spirolide. Control cells were incubated with vehicle in the absence of toxin. Results are the average of two experiments performed in duplicate. Following incubation cytotoxicity was assessed using measures of mitochondrial respiration (MTS assay), plasma membrane integrity (LDH release), and lysosomal function (NR staining). Results were normalized to those of control wells.