Skip to main content
. 2011 Dec 30;4(1):1–14. doi: 10.3390/toxins4010001

Figure 2.

Figure 2

Phosphorylation of PKB and MAPK by nicotinic agonists is inhibited by 13dmC. Fully differentiated C2C12 myotubes were pre-treated with 13dmC (100 nM; “+”) or vehicle (methanol + 0.1% trifluoracetic acid; “−”) for 30 min. Cells were then treated with: nicotine (10 μM; panel a), PHA543613 (PHA, 10 µM; panel b), or insulin (100 nM; panel c) for 20 min. Corresponding controls received vehicle alone. Cell lysates were analyzed by immunoblot for p-473 PKB (MW 60 kDa) and p-MAPK (MW 42 and 44 kDa) using phosphospecific antibodies. These are representative blots from 2–3 independent experiments.