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Sensitivity of the phosphoenzyme to ADP. Following phosphorylation with [γ-32P]ATP in SPM at 0 °C, enzyme reconstituted in PC (circles) or 90PC:10PS (triangles) was treated with excess unlabeled ATP (filled symbols) or ADP (open symbols) for the indicated times. The amplitude of the rapid phase of the ADP-induced decay reflects E1P, whereas the slow phase corresponds to E2P (E2P fraction indicated in panels). Refer to Table S1 for statistical analysis.
