Fig. 5.
The effect of cellular dilution on association rate constants. Dilution by microinjection was calculated as the ratio of CyTEM intensity before and after injection. (A) Dilution versus association rate constants in single cells. (B) Effect of low (1–1.4) and high (1.4–4) dilutions following PBS injections on t1/2 of single cells as determined by FRAP. (C) Effect of low and high dilutions on association rate constants (data from A). The ratio of t1/2 before and after injection was determined for each cell, and the average ratio for low- and high-diluted cells is shown in the inset. Error bars represent the standard error of the mean.