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Association rate constants of CyTEM-YBLIP in vitro, in cell extract, and in cells
| Interacting pair | Association rate constant, × 105 M-1 s-1 | |||
| CyTEM |
YBLIP |
In vitro |
Cell extract |
In cell |
| WT | WT | 3.0 ± 0.4 | 2.3 ± 0.4 | 1.9 ± 0.1 |
| WT | D163K | 9.9 ± 0.4 | 6.2 ± 0.4 | 4.7 ± 0.3 |
| R243A | WT | 0.54 ± 0.1 | 0.52 ± 0.04 | |
Values are reported as mean ± SEM. Number of cells analyzed for in cell rates is 25 for WT, 36 for D163K, and 20 for R243A.
