Fig. 3.

Unique phosphotyrosine signatures are observed in a mouse model of prostate cancer progression. (A) Heatmap representing unique clusters of tyrosine phosphorylation for each mouse tumor phenotype. Each row corresponds to a unique phosphopeptide. Red, hyperphosphorylation; green, hypophosphorylation for each phosphopeptide. (B) Specific tyrosine kinases are observed in an oncogene-specific fashion. Signal-to-noise ratio (SNR) (relative to AKT) was calculated for each phosphorylation event and plotted. Positive SNR confirms elevation of that particular phosphorylation event. Western blotting validates indicated oncogene-specific phosphorylation results.