Fig. 4.

Altered skeletal muscle energy expenditure in dKO mice. (A) Histological sections of TA isolated from 7-wk-old control and dKO mice were analyzed by metachromatic ATPase, SDH, and NADH staining. (Scale bars, 100 μm for metachromatic ATPase, 40 μm for SDH and NADH staining.) (B) Real-time RT-PCR analysis of different Myh genes, Ppargc1a, Ppargc1b, Acadm, and Ucp2 in control (Ctrl) and dKO mice, at 7 wk of age. Data are expressed as mean ± SEM. n = 4. *P < 0.05. dKO mice show significantly higher (C) O₂ consumption, (D) CO₂ production, and (E) heat production than control mice, between 5 and 7 wk of age. *P < 0.05. Values are mean ± SEM. n = 7.