Fig. 3.

The dwell time of RecQ on ssDNA increases with oligonucleotide length. (A) Kinetic traces for the dissociation of RecQ-ssDNA complexes, measured by fluorescence, for oligonucleotides of varying lengths. The traces were each fit to Eq. 4 (black lines). The amplitudes are the same for all substrates. (B) The reciprocal of the rate constants determined from (A) are plotted as a function of DNA length. The solid line is a fit of the initial linear region (up to 60 nt); the slope is 0.019 ± 0.001 s/nt.